Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and
Download scientific diagram | Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and CLIP-tag fused to the protein of interest bind benzylguanine-or benzylcytosine-substrates, respectively. There are various cell-permeable fluorescent substrates and a non-fluorescent substrate available. (b) For labeling of the whole fusion protein pool cells are incubated with a fluorescent substrate, washed and fixed/imaged. (c) For age-dependent labelings cells are first incubated with the non-fluorescent blocker followed by labeling of the newly-synthesized fusion protein with a fluorescent substrate. To omit continuous labeling with the first substrate additional blocking/ labeling steps can be added. from publication: A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags | Correlative light and electron microscopy (CLEM) is a powerful approach to investigate the molecular ultrastructure of labeled cell compartments. However, quantitative CLEM studies are rare, mainly due to small sample sizes and the sensitivity of fluorescent proteins to | Protein Tagging, Electron Microscopy and Correlative Light Electron Microscopy | ResearchGate, the professional network for scientists.
SNAP/CLIP-Tags and Strain-Promoted Azide–Alkyne Cycloaddition (SPAAC)/Inverse Electron Demand Diels–Alder (IEDDA) for Intracellular Orthogonal/Bioorthogonal Labeling
Degradation of old insulin SGs in MGBs. (a) SIM image SIM image of
SNAP-tag - Wikipedia
IJMS, Free Full-Text
Anna IVANOVA, PhD, Molecular diabetology
SNAP-tag fluorogenic probes for wash free protein labeling - ScienceDirect
SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos
A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags
Multivalent Chelators for In Vivo Protein Labeling - Wieneke - 2019 - Angewandte Chemie International Edition - Wiley Online Library
